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Formulation:Article Title: Impacts of fast production of afucosylated antibodies and Fc mutants in ExpiCHO-S™ for enhancing FcγRIIIa binding and NK cell activation. Article Snippet: The resulting heat capacity was baseline corrected by subtracting against a blank PBS scan and fitted with the non-2 state transitions function using Article Title: A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal. Article Snippet: Accepted Manuscript A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal Wen-Si Yang, Yuan Hong, Ying Zhang, Da-Cheng Wang, De-Feng Li, Yan-Jie Hou PII: S0006-291X(18)30374-7 DOI: 10.1016/j.bbrc.2018.02.143 Reference: YBBRC 39508 To appear in: Biochemical and Biophysical Research Communications Received Date: 14 February 2018 Accepted Date: 16 February 2018 Please cite this article as: W.-S. Yang, Y. Hong, Y. Zhang, D.-C. Wang, D.-F. Li, Y.-J.. Hou, A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal, Biochemical and Biophysical Research Communications (2018), doi: 10.1016/j.bbrc.2018.02.143.. This is a PDF file of an unedited manuscript that has been accepted for publication. Article Title: Effects of dehydroepiandrosterone sulfate on the evoked cortical activity of controls and of brain-injured rats. Article Snippet: Dehydroepiandrosterone (DHEA) and its sulfate (DHEAS) are sex hormone precursors which exert marked neurotrophic and/or neuroprotective activity in the central nervous system (CNS).. In the present electrophysiological experiments, we studied the effects of peripherally administered DHEAS on responses of the primary somatosensory (SSI) and motor cortices (MI) of (i) anesthetized controls and (ii) MI focal cold-lesioned rats. (iii) The effects of DHEAS on the field excitatory postsynaptic potentials (fEPSPs) were also studied in vitro brain slices.. DHEAS (50 mg/kg) was injected subcutaneously 12 h before and immediately after cold lesion induction. Article Title: Dissociation of MIF-rpS3 complex and sequential NF-κB activation is involved in IR-induced metastatic conversion of NSCLC. Article Snippet: Frequent relapse and spreading of tumors during radiotherapy are principal obstacles to treatment of non-small cell lung cancer (NSCLC).. In this study, we aimed to investigate how macrophage migration inhibitory factor (MIF) which is expressed at high levels in metastatic and primary lung cancer cells could regulate NSCLC metastasis in response to ionizing radiation (IR).. The results indicated that MIF and ribosomal protein S3 (rpS3) were shown to be connected to inflammation, proliferation, and metastasis of NSCLC via IR-induced activation of the NF-kB pathway. Article Title: A ligand-induced structural change in fatty acid–binding protein 1 is associated with potentiation of peroxisome proliferator–activated receptor α agonists Article Snippet: The data were processed using the Article Title: Structural and Functional Properties of Tropomyosin Isoforms Tpm4.1 and Tpm2.1 Article Snippet: Tropomyosin (Tpm) is one of the most important partners of actin filament that largely determines its properties.. In animal organisms, there are different isoforms of Tpm, which are believed to be involved in the regulation of various cellular functions.. However, molecular mechanisms by which various Tpm cytoplasmic regulate of the functioning of actin filaments are still poorly understood. Article Title: Capped and uncapped antibody cysteines, and their use in antibody-drug conjugation Article Snippet: The resulting heat capacity was baseline corrected by subtracting against a blank Histidine/sucrose formulation scan and fitted with the non-2 state transitions function using Article Title: The interaction of hyperthermophilic TATA-box binding protein with single-stranded DNA is entropically favorable and exhibits a large negative heat capacity change at high salt concentration. Article Snippet: Molecular BioSystems This article was published as part of the 2009 Emerging Investigators Issue Highlighting the work of outstanding young scientists at the chemical- and systems-biology interfaces Please take a look at the full table of contents to access the other papers in this issue.. Pu bl is he d on 1 3 M ay 2 00 9.. D ow nl oa de d by U ni ve rs ity o f W in ds or o n 28 /1 0/ 20 14 0 3: 43 :1 6. Software:Article Title: Impacts of fast production of afucosylated antibodies and Fc mutants in ExpiCHO-S™ for enhancing FcγRIIIa binding and NK cell activation. Article Snippet: The resulting heat capacity was baseline corrected by subtracting against a blank PBS scan and fitted with the non-2 state transitions function using Article Title: A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal. Article Snippet: Accepted Manuscript A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal Wen-Si Yang, Yuan Hong, Ying Zhang, Da-Cheng Wang, De-Feng Li, Yan-Jie Hou PII: S0006-291X(18)30374-7 DOI: 10.1016/j.bbrc.2018.02.143 Reference: YBBRC 39508 To appear in: Biochemical and Biophysical Research Communications Received Date: 14 February 2018 Accepted Date: 16 February 2018 Please cite this article as: W.-S. Yang, Y. Hong, Y. Zhang, D.-C. Wang, D.-F. Li, Y.-J.. Hou, A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal, Biochemical and Biophysical Research Communications (2018), doi: 10.1016/j.bbrc.2018.02.143.. This is a PDF file of an unedited manuscript that has been accepted for publication. Article Title: Effects of dehydroepiandrosterone sulfate on the evoked cortical activity of controls and of brain-injured rats. Article Snippet: Dehydroepiandrosterone (DHEA) and its sulfate (DHEAS) are sex hormone precursors which exert marked neurotrophic and/or neuroprotective activity in the central nervous system (CNS).. In the present electrophysiological experiments, we studied the effects of peripherally administered DHEAS on responses of the primary somatosensory (SSI) and motor cortices (MI) of (i) anesthetized controls and (ii) MI focal cold-lesioned rats. (iii) The effects of DHEAS on the field excitatory postsynaptic potentials (fEPSPs) were also studied in vitro brain slices.. DHEAS (50 mg/kg) was injected subcutaneously 12 h before and immediately after cold lesion induction. Article Title: Dissociation of MIF-rpS3 complex and sequential NF-κB activation is involved in IR-induced metastatic conversion of NSCLC. Article Snippet: Frequent relapse and spreading of tumors during radiotherapy are principal obstacles to treatment of non-small cell lung cancer (NSCLC).. In this study, we aimed to investigate how macrophage migration inhibitory factor (MIF) which is expressed at high levels in metastatic and primary lung cancer cells could regulate NSCLC metastasis in response to ionizing radiation (IR).. The results indicated that MIF and ribosomal protein S3 (rpS3) were shown to be connected to inflammation, proliferation, and metastasis of NSCLC via IR-induced activation of the NF-kB pathway. Article Title: A ligand-induced structural change in fatty acid–binding protein 1 is associated with potentiation of peroxisome proliferator–activated receptor α agonists Article Snippet: The data were processed using the Article Title: Structural and Functional Properties of Tropomyosin Isoforms Tpm4.1 and Tpm2.1 Article Snippet: Tropomyosin (Tpm) is one of the most important partners of actin filament that largely determines its properties.. In animal organisms, there are different isoforms of Tpm, which are believed to be involved in the regulation of various cellular functions.. However, molecular mechanisms by which various Tpm cytoplasmic regulate of the functioning of actin filaments are still poorly understood. Article Title: Capped and uncapped antibody cysteines, and their use in antibody-drug conjugation Article Snippet: The resulting heat capacity was baseline corrected by subtracting against a blank Histidine/sucrose formulation scan and fitted with the non-2 state transitions function using Article Title: The interaction of hyperthermophilic TATA-box binding protein with single-stranded DNA is entropically favorable and exhibits a large negative heat capacity change at high salt concentration. Article Snippet: Molecular BioSystems This article was published as part of the 2009 Emerging Investigators Issue Highlighting the work of outstanding young scientists at the chemical- and systems-biology interfaces Please take a look at the full table of contents to access the other papers in this issue.. Pu bl is he d on 1 3 M ay 2 00 9.. D ow nl oa de d by U ni ve rs ity o f W in ds or o n 28 /1 0/ 20 14 0 3: 43 :1 6. Binding Assay:Article Title: Impacts of fast production of afucosylated antibodies and Fc mutants in ExpiCHO-S™ for enhancing FcγRIIIa binding and NK cell activation. Article Snippet: The resulting heat capacity was baseline corrected by subtracting against a blank PBS scan and fitted with the non-2 state transitions function using Article Title: A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal. Article Snippet: Accepted Manuscript A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal Wen-Si Yang, Yuan Hong, Ying Zhang, Da-Cheng Wang, De-Feng Li, Yan-Jie Hou PII: S0006-291X(18)30374-7 DOI: 10.1016/j.bbrc.2018.02.143 Reference: YBBRC 39508 To appear in: Biochemical and Biophysical Research Communications Received Date: 14 February 2018 Accepted Date: 16 February 2018 Please cite this article as: W.-S. Yang, Y. Hong, Y. Zhang, D.-C. Wang, D.-F. Li, Y.-J.. Hou, A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal, Biochemical and Biophysical Research Communications (2018), doi: 10.1016/j.bbrc.2018.02.143.. This is a PDF file of an unedited manuscript that has been accepted for publication. Article Title: Effects of dehydroepiandrosterone sulfate on the evoked cortical activity of controls and of brain-injured rats. Article Snippet: Dehydroepiandrosterone (DHEA) and its sulfate (DHEAS) are sex hormone precursors which exert marked neurotrophic and/or neuroprotective activity in the central nervous system (CNS).. In the present electrophysiological experiments, we studied the effects of peripherally administered DHEAS on responses of the primary somatosensory (SSI) and motor cortices (MI) of (i) anesthetized controls and (ii) MI focal cold-lesioned rats. (iii) The effects of DHEAS on the field excitatory postsynaptic potentials (fEPSPs) were also studied in vitro brain slices.. DHEAS (50 mg/kg) was injected subcutaneously 12 h before and immediately after cold lesion induction. Article Title: Dissociation of MIF-rpS3 complex and sequential NF-κB activation is involved in IR-induced metastatic conversion of NSCLC. Article Snippet: Frequent relapse and spreading of tumors during radiotherapy are principal obstacles to treatment of non-small cell lung cancer (NSCLC).. In this study, we aimed to investigate how macrophage migration inhibitory factor (MIF) which is expressed at high levels in metastatic and primary lung cancer cells could regulate NSCLC metastasis in response to ionizing radiation (IR).. The results indicated that MIF and ribosomal protein S3 (rpS3) were shown to be connected to inflammation, proliferation, and metastasis of NSCLC via IR-induced activation of the NF-kB pathway. Article Title: A ligand-induced structural change in fatty acid–binding protein 1 is associated with potentiation of peroxisome proliferator–activated receptor α agonists Article Snippet: The data were processed using the Article Title: Structural and Functional Properties of Tropomyosin Isoforms Tpm4.1 and Tpm2.1 Article Snippet: Tropomyosin (Tpm) is one of the most important partners of actin filament that largely determines its properties.. In animal organisms, there are different isoforms of Tpm, which are believed to be involved in the regulation of various cellular functions.. However, molecular mechanisms by which various Tpm cytoplasmic regulate of the functioning of actin filaments are still poorly understood. Article Title: Capped and uncapped antibody cysteines, and their use in antibody-drug conjugation Article Snippet: The resulting heat capacity was baseline corrected by subtracting against a blank Histidine/sucrose formulation scan and fitted with the non-2 state transitions function using Article Title: The interaction of hyperthermophilic TATA-box binding protein with single-stranded DNA is entropically favorable and exhibits a large negative heat capacity change at high salt concentration. Article Snippet: Molecular BioSystems This article was published as part of the 2009 Emerging Investigators Issue Highlighting the work of outstanding young scientists at the chemical- and systems-biology interfaces Please take a look at the full table of contents to access the other papers in this issue.. Pu bl is he d on 1 3 M ay 2 00 9.. D ow nl oa de d by U ni ve rs ity o f W in ds or o n 28 /1 0/ 20 14 0 3: 43 :1 6. Nuclear Magnetic Resonance:Article Title: Impacts of fast production of afucosylated antibodies and Fc mutants in ExpiCHO-S™ for enhancing FcγRIIIa binding and NK cell activation. Article Snippet: The resulting heat capacity was baseline corrected by subtracting against a blank PBS scan and fitted with the non-2 state transitions function using Article Title: A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal. Article Snippet: Accepted Manuscript A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal Wen-Si Yang, Yuan Hong, Ying Zhang, Da-Cheng Wang, De-Feng Li, Yan-Jie Hou PII: S0006-291X(18)30374-7 DOI: 10.1016/j.bbrc.2018.02.143 Reference: YBBRC 39508 To appear in: Biochemical and Biophysical Research Communications Received Date: 14 February 2018 Accepted Date: 16 February 2018 Please cite this article as: W.-S. Yang, Y. Hong, Y. Zhang, D.-C. Wang, D.-F. Li, Y.-J.. Hou, A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal, Biochemical and Biophysical Research Communications (2018), doi: 10.1016/j.bbrc.2018.02.143.. This is a PDF file of an unedited manuscript that has been accepted for publication. Article Title: Effects of dehydroepiandrosterone sulfate on the evoked cortical activity of controls and of brain-injured rats. Article Snippet: Dehydroepiandrosterone (DHEA) and its sulfate (DHEAS) are sex hormone precursors which exert marked neurotrophic and/or neuroprotective activity in the central nervous system (CNS).. In the present electrophysiological experiments, we studied the effects of peripherally administered DHEAS on responses of the primary somatosensory (SSI) and motor cortices (MI) of (i) anesthetized controls and (ii) MI focal cold-lesioned rats. (iii) The effects of DHEAS on the field excitatory postsynaptic potentials (fEPSPs) were also studied in vitro brain slices.. DHEAS (50 mg/kg) was injected subcutaneously 12 h before and immediately after cold lesion induction. Article Title: Dissociation of MIF-rpS3 complex and sequential NF-κB activation is involved in IR-induced metastatic conversion of NSCLC. Article Snippet: Frequent relapse and spreading of tumors during radiotherapy are principal obstacles to treatment of non-small cell lung cancer (NSCLC).. In this study, we aimed to investigate how macrophage migration inhibitory factor (MIF) which is expressed at high levels in metastatic and primary lung cancer cells could regulate NSCLC metastasis in response to ionizing radiation (IR).. The results indicated that MIF and ribosomal protein S3 (rpS3) were shown to be connected to inflammation, proliferation, and metastasis of NSCLC via IR-induced activation of the NF-kB pathway. Article Title: A ligand-induced structural change in fatty acid–binding protein 1 is associated with potentiation of peroxisome proliferator–activated receptor α agonists Article Snippet: The data were processed using the Article Title: Structural and Functional Properties of Tropomyosin Isoforms Tpm4.1 and Tpm2.1 Article Snippet: Tropomyosin (Tpm) is one of the most important partners of actin filament that largely determines its properties.. In animal organisms, there are different isoforms of Tpm, which are believed to be involved in the regulation of various cellular functions.. However, molecular mechanisms by which various Tpm cytoplasmic regulate of the functioning of actin filaments are still poorly understood. Article Title: Capped and uncapped antibody cysteines, and their use in antibody-drug conjugation Article Snippet: The resulting heat capacity was baseline corrected by subtracting against a blank Histidine/sucrose formulation scan and fitted with the non-2 state transitions function using Article Title: The interaction of hyperthermophilic TATA-box binding protein with single-stranded DNA is entropically favorable and exhibits a large negative heat capacity change at high salt concentration. Article Snippet: Molecular BioSystems This article was published as part of the 2009 Emerging Investigators Issue Highlighting the work of outstanding young scientists at the chemical- and systems-biology interfaces Please take a look at the full table of contents to access the other papers in this issue.. Pu bl is he d on 1 3 M ay 2 00 9.. D ow nl oa de d by U ni ve rs ity o f W in ds or o n 28 /1 0/ 20 14 0 3: 43 :1 6. Sample Prep:Article Title: Impacts of fast production of afucosylated antibodies and Fc mutants in ExpiCHO-S™ for enhancing FcγRIIIa binding and NK cell activation. Article Snippet: The resulting heat capacity was baseline corrected by subtracting against a blank PBS scan and fitted with the non-2 state transitions function using Article Title: A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal. Article Snippet: Accepted Manuscript A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal Wen-Si Yang, Yuan Hong, Ying Zhang, Da-Cheng Wang, De-Feng Li, Yan-Jie Hou PII: S0006-291X(18)30374-7 DOI: 10.1016/j.bbrc.2018.02.143 Reference: YBBRC 39508 To appear in: Biochemical and Biophysical Research Communications Received Date: 14 February 2018 Accepted Date: 16 February 2018 Please cite this article as: W.-S. Yang, Y. Hong, Y. Zhang, D.-C. Wang, D.-F. Li, Y.-J.. Hou, A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal, Biochemical and Biophysical Research Communications (2018), doi: 10.1016/j.bbrc.2018.02.143.. This is a PDF file of an unedited manuscript that has been accepted for publication. Article Title: Effects of dehydroepiandrosterone sulfate on the evoked cortical activity of controls and of brain-injured rats. Article Snippet: Dehydroepiandrosterone (DHEA) and its sulfate (DHEAS) are sex hormone precursors which exert marked neurotrophic and/or neuroprotective activity in the central nervous system (CNS).. In the present electrophysiological experiments, we studied the effects of peripherally administered DHEAS on responses of the primary somatosensory (SSI) and motor cortices (MI) of (i) anesthetized controls and (ii) MI focal cold-lesioned rats. (iii) The effects of DHEAS on the field excitatory postsynaptic potentials (fEPSPs) were also studied in vitro brain slices.. DHEAS (50 mg/kg) was injected subcutaneously 12 h before and immediately after cold lesion induction. Article Title: Dissociation of MIF-rpS3 complex and sequential NF-κB activation is involved in IR-induced metastatic conversion of NSCLC. Article Snippet: Frequent relapse and spreading of tumors during radiotherapy are principal obstacles to treatment of non-small cell lung cancer (NSCLC).. In this study, we aimed to investigate how macrophage migration inhibitory factor (MIF) which is expressed at high levels in metastatic and primary lung cancer cells could regulate NSCLC metastasis in response to ionizing radiation (IR).. The results indicated that MIF and ribosomal protein S3 (rpS3) were shown to be connected to inflammation, proliferation, and metastasis of NSCLC via IR-induced activation of the NF-kB pathway. Article Title: A ligand-induced structural change in fatty acid–binding protein 1 is associated with potentiation of peroxisome proliferator–activated receptor α agonists Article Snippet: The data were processed using the Article Title: Structural and Functional Properties of Tropomyosin Isoforms Tpm4.1 and Tpm2.1 Article Snippet: Tropomyosin (Tpm) is one of the most important partners of actin filament that largely determines its properties.. In animal organisms, there are different isoforms of Tpm, which are believed to be involved in the regulation of various cellular functions.. However, molecular mechanisms by which various Tpm cytoplasmic regulate of the functioning of actin filaments are still poorly understood. Article Title: Capped and uncapped antibody cysteines, and their use in antibody-drug conjugation Article Snippet: The resulting heat capacity was baseline corrected by subtracting against a blank Histidine/sucrose formulation scan and fitted with the non-2 state transitions function using Article Title: The interaction of hyperthermophilic TATA-box binding protein with single-stranded DNA is entropically favorable and exhibits a large negative heat capacity change at high salt concentration. Article Snippet: Molecular BioSystems This article was published as part of the 2009 Emerging Investigators Issue Highlighting the work of outstanding young scientists at the chemical- and systems-biology interfaces Please take a look at the full table of contents to access the other papers in this issue.. Pu bl is he d on 1 3 M ay 2 00 9.. D ow nl oa de d by U ni ve rs ity o f W in ds or o n 28 /1 0/ 20 14 0 3: 43 :1 6. Titration:Article Title: Impacts of fast production of afucosylated antibodies and Fc mutants in ExpiCHO-S™ for enhancing FcγRIIIa binding and NK cell activation. Article Snippet: The resulting heat capacity was baseline corrected by subtracting against a blank PBS scan and fitted with the non-2 state transitions function using Article Title: A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal. Article Snippet: Accepted Manuscript A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal Wen-Si Yang, Yuan Hong, Ying Zhang, Da-Cheng Wang, De-Feng Li, Yan-Jie Hou PII: S0006-291X(18)30374-7 DOI: 10.1016/j.bbrc.2018.02.143 Reference: YBBRC 39508 To appear in: Biochemical and Biophysical Research Communications Received Date: 14 February 2018 Accepted Date: 16 February 2018 Please cite this article as: W.-S. Yang, Y. Hong, Y. Zhang, D.-C. Wang, D.-F. Li, Y.-J.. Hou, A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal, Biochemical and Biophysical Research Communications (2018), doi: 10.1016/j.bbrc.2018.02.143.. This is a PDF file of an unedited manuscript that has been accepted for publication. Article Title: Effects of dehydroepiandrosterone sulfate on the evoked cortical activity of controls and of brain-injured rats. Article Snippet: Dehydroepiandrosterone (DHEA) and its sulfate (DHEAS) are sex hormone precursors which exert marked neurotrophic and/or neuroprotective activity in the central nervous system (CNS).. In the present electrophysiological experiments, we studied the effects of peripherally administered DHEAS on responses of the primary somatosensory (SSI) and motor cortices (MI) of (i) anesthetized controls and (ii) MI focal cold-lesioned rats. (iii) The effects of DHEAS on the field excitatory postsynaptic potentials (fEPSPs) were also studied in vitro brain slices.. DHEAS (50 mg/kg) was injected subcutaneously 12 h before and immediately after cold lesion induction. Article Title: Dissociation of MIF-rpS3 complex and sequential NF-κB activation is involved in IR-induced metastatic conversion of NSCLC. Article Snippet: Frequent relapse and spreading of tumors during radiotherapy are principal obstacles to treatment of non-small cell lung cancer (NSCLC).. In this study, we aimed to investigate how macrophage migration inhibitory factor (MIF) which is expressed at high levels in metastatic and primary lung cancer cells could regulate NSCLC metastasis in response to ionizing radiation (IR).. The results indicated that MIF and ribosomal protein S3 (rpS3) were shown to be connected to inflammation, proliferation, and metastasis of NSCLC via IR-induced activation of the NF-kB pathway. Article Title: A ligand-induced structural change in fatty acid–binding protein 1 is associated with potentiation of peroxisome proliferator–activated receptor α agonists Article Snippet: The data were processed using the Article Title: Structural and Functional Properties of Tropomyosin Isoforms Tpm4.1 and Tpm2.1 Article Snippet: Tropomyosin (Tpm) is one of the most important partners of actin filament that largely determines its properties.. In animal organisms, there are different isoforms of Tpm, which are believed to be involved in the regulation of various cellular functions.. However, molecular mechanisms by which various Tpm cytoplasmic regulate of the functioning of actin filaments are still poorly understood. Article Title: Capped and uncapped antibody cysteines, and their use in antibody-drug conjugation Article Snippet: The resulting heat capacity was baseline corrected by subtracting against a blank Histidine/sucrose formulation scan and fitted with the non-2 state transitions function using Article Title: The interaction of hyperthermophilic TATA-box binding protein with single-stranded DNA is entropically favorable and exhibits a large negative heat capacity change at high salt concentration. Article Snippet: Molecular BioSystems This article was published as part of the 2009 Emerging Investigators Issue Highlighting the work of outstanding young scientists at the chemical- and systems-biology interfaces Please take a look at the full table of contents to access the other papers in this issue.. Pu bl is he d on 1 3 M ay 2 00 9.. D ow nl oa de d by U ni ve rs ity o f W in ds or o n 28 /1 0/ 20 14 0 3: 43 :1 6. Bimolecular Fluorescence Complementation Assay:Article Title: Impacts of fast production of afucosylated antibodies and Fc mutants in ExpiCHO-S™ for enhancing FcγRIIIa binding and NK cell activation. Article Snippet: The resulting heat capacity was baseline corrected by subtracting against a blank PBS scan and fitted with the non-2 state transitions function using Article Title: A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal. Article Snippet: Accepted Manuscript A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal Wen-Si Yang, Yuan Hong, Ying Zhang, Da-Cheng Wang, De-Feng Li, Yan-Jie Hou PII: S0006-291X(18)30374-7 DOI: 10.1016/j.bbrc.2018.02.143 Reference: YBBRC 39508 To appear in: Biochemical and Biophysical Research Communications Received Date: 14 February 2018 Accepted Date: 16 February 2018 Please cite this article as: W.-S. Yang, Y. Hong, Y. Zhang, D.-C. Wang, D.-F. Li, Y.-J.. Hou, A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal, Biochemical and Biophysical Research Communications (2018), doi: 10.1016/j.bbrc.2018.02.143.. This is a PDF file of an unedited manuscript that has been accepted for publication. Article Title: Effects of dehydroepiandrosterone sulfate on the evoked cortical activity of controls and of brain-injured rats. Article Snippet: Dehydroepiandrosterone (DHEA) and its sulfate (DHEAS) are sex hormone precursors which exert marked neurotrophic and/or neuroprotective activity in the central nervous system (CNS).. In the present electrophysiological experiments, we studied the effects of peripherally administered DHEAS on responses of the primary somatosensory (SSI) and motor cortices (MI) of (i) anesthetized controls and (ii) MI focal cold-lesioned rats. (iii) The effects of DHEAS on the field excitatory postsynaptic potentials (fEPSPs) were also studied in vitro brain slices.. DHEAS (50 mg/kg) was injected subcutaneously 12 h before and immediately after cold lesion induction. Article Title: Dissociation of MIF-rpS3 complex and sequential NF-κB activation is involved in IR-induced metastatic conversion of NSCLC. Article Snippet: Frequent relapse and spreading of tumors during radiotherapy are principal obstacles to treatment of non-small cell lung cancer (NSCLC).. In this study, we aimed to investigate how macrophage migration inhibitory factor (MIF) which is expressed at high levels in metastatic and primary lung cancer cells could regulate NSCLC metastasis in response to ionizing radiation (IR).. The results indicated that MIF and ribosomal protein S3 (rpS3) were shown to be connected to inflammation, proliferation, and metastasis of NSCLC via IR-induced activation of the NF-kB pathway. Article Title: A ligand-induced structural change in fatty acid–binding protein 1 is associated with potentiation of peroxisome proliferator–activated receptor α agonists Article Snippet: The data were processed using the Article Title: Structural and Functional Properties of Tropomyosin Isoforms Tpm4.1 and Tpm2.1 Article Snippet: Tropomyosin (Tpm) is one of the most important partners of actin filament that largely determines its properties.. In animal organisms, there are different isoforms of Tpm, which are believed to be involved in the regulation of various cellular functions.. However, molecular mechanisms by which various Tpm cytoplasmic regulate of the functioning of actin filaments are still poorly understood. Article Title: Capped and uncapped antibody cysteines, and their use in antibody-drug conjugation Article Snippet: The resulting heat capacity was baseline corrected by subtracting against a blank Histidine/sucrose formulation scan and fitted with the non-2 state transitions function using Article Title: The interaction of hyperthermophilic TATA-box binding protein with single-stranded DNA is entropically favorable and exhibits a large negative heat capacity change at high salt concentration. Article Snippet: Molecular BioSystems This article was published as part of the 2009 Emerging Investigators Issue Highlighting the work of outstanding young scientists at the chemical- and systems-biology interfaces Please take a look at the full table of contents to access the other papers in this issue.. Pu bl is he d on 1 3 M ay 2 00 9.. D ow nl oa de d by U ni ve rs ity o f W in ds or o n 28 /1 0/ 20 14 0 3: 43 :1 6. Construct:Article Title: Impacts of fast production of afucosylated antibodies and Fc mutants in ExpiCHO-S™ for enhancing FcγRIIIa binding and NK cell activation. Article Snippet: The resulting heat capacity was baseline corrected by subtracting against a blank PBS scan and fitted with the non-2 state transitions function using Article Title: A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal. Article Snippet: Accepted Manuscript A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal Wen-Si Yang, Yuan Hong, Ying Zhang, Da-Cheng Wang, De-Feng Li, Yan-Jie Hou PII: S0006-291X(18)30374-7 DOI: 10.1016/j.bbrc.2018.02.143 Reference: YBBRC 39508 To appear in: Biochemical and Biophysical Research Communications Received Date: 14 February 2018 Accepted Date: 16 February 2018 Please cite this article as: W.-S. Yang, Y. Hong, Y. Zhang, D.-C. Wang, D.-F. Li, Y.-J.. Hou, A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal, Biochemical and Biophysical Research Communications (2018), doi: 10.1016/j.bbrc.2018.02.143.. This is a PDF file of an unedited manuscript that has been accepted for publication. Article Title: Effects of dehydroepiandrosterone sulfate on the evoked cortical activity of controls and of brain-injured rats. Article Snippet: Dehydroepiandrosterone (DHEA) and its sulfate (DHEAS) are sex hormone precursors which exert marked neurotrophic and/or neuroprotective activity in the central nervous system (CNS).. In the present electrophysiological experiments, we studied the effects of peripherally administered DHEAS on responses of the primary somatosensory (SSI) and motor cortices (MI) of (i) anesthetized controls and (ii) MI focal cold-lesioned rats. (iii) The effects of DHEAS on the field excitatory postsynaptic potentials (fEPSPs) were also studied in vitro brain slices.. DHEAS (50 mg/kg) was injected subcutaneously 12 h before and immediately after cold lesion induction. Article Title: Dissociation of MIF-rpS3 complex and sequential NF-κB activation is involved in IR-induced metastatic conversion of NSCLC. Article Snippet: Frequent relapse and spreading of tumors during radiotherapy are principal obstacles to treatment of non-small cell lung cancer (NSCLC).. In this study, we aimed to investigate how macrophage migration inhibitory factor (MIF) which is expressed at high levels in metastatic and primary lung cancer cells could regulate NSCLC metastasis in response to ionizing radiation (IR).. The results indicated that MIF and ribosomal protein S3 (rpS3) were shown to be connected to inflammation, proliferation, and metastasis of NSCLC via IR-induced activation of the NF-kB pathway. Article Title: A ligand-induced structural change in fatty acid–binding protein 1 is associated with potentiation of peroxisome proliferator–activated receptor α agonists Article Snippet: The data were processed using the Article Title: Structural and Functional Properties of Tropomyosin Isoforms Tpm4.1 and Tpm2.1 Article Snippet: Tropomyosin (Tpm) is one of the most important partners of actin filament that largely determines its properties.. In animal organisms, there are different isoforms of Tpm, which are believed to be involved in the regulation of various cellular functions.. However, molecular mechanisms by which various Tpm cytoplasmic regulate of the functioning of actin filaments are still poorly understood. Article Title: Capped and uncapped antibody cysteines, and their use in antibody-drug conjugation Article Snippet: The resulting heat capacity was baseline corrected by subtracting against a blank Histidine/sucrose formulation scan and fitted with the non-2 state transitions function using Article Title: The interaction of hyperthermophilic TATA-box binding protein with single-stranded DNA is entropically favorable and exhibits a large negative heat capacity change at high salt concentration. Article Snippet: Molecular BioSystems This article was published as part of the 2009 Emerging Investigators Issue Highlighting the work of outstanding young scientists at the chemical- and systems-biology interfaces Please take a look at the full table of contents to access the other papers in this issue.. Pu bl is he d on 1 3 M ay 2 00 9.. D ow nl oa de d by U ni ve rs ity o f W in ds or o n 28 /1 0/ 20 14 0 3: 43 :1 6. Amplification:Article Title: Impacts of fast production of afucosylated antibodies and Fc mutants in ExpiCHO-S™ for enhancing FcγRIIIa binding and NK cell activation. Article Snippet: The resulting heat capacity was baseline corrected by subtracting against a blank PBS scan and fitted with the non-2 state transitions function using Article Title: A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal. Article Snippet: Accepted Manuscript A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal Wen-Si Yang, Yuan Hong, Ying Zhang, Da-Cheng Wang, De-Feng Li, Yan-Jie Hou PII: S0006-291X(18)30374-7 DOI: 10.1016/j.bbrc.2018.02.143 Reference: YBBRC 39508 To appear in: Biochemical and Biophysical Research Communications Received Date: 14 February 2018 Accepted Date: 16 February 2018 Please cite this article as: W.-S. Yang, Y. Hong, Y. Zhang, D.-C. Wang, D.-F. Li, Y.-J.. Hou, A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal, Biochemical and Biophysical Research Communications (2018), doi: 10.1016/j.bbrc.2018.02.143.. This is a PDF file of an unedited manuscript that has been accepted for publication. Article Title: Effects of dehydroepiandrosterone sulfate on the evoked cortical activity of controls and of brain-injured rats. Article Snippet: Dehydroepiandrosterone (DHEA) and its sulfate (DHEAS) are sex hormone precursors which exert marked neurotrophic and/or neuroprotective activity in the central nervous system (CNS).. In the present electrophysiological experiments, we studied the effects of peripherally administered DHEAS on responses of the primary somatosensory (SSI) and motor cortices (MI) of (i) anesthetized controls and (ii) MI focal cold-lesioned rats. (iii) The effects of DHEAS on the field excitatory postsynaptic potentials (fEPSPs) were also studied in vitro brain slices.. DHEAS (50 mg/kg) was injected subcutaneously 12 h before and immediately after cold lesion induction. Article Title: Dissociation of MIF-rpS3 complex and sequential NF-κB activation is involved in IR-induced metastatic conversion of NSCLC. Article Snippet: Frequent relapse and spreading of tumors during radiotherapy are principal obstacles to treatment of non-small cell lung cancer (NSCLC).. In this study, we aimed to investigate how macrophage migration inhibitory factor (MIF) which is expressed at high levels in metastatic and primary lung cancer cells could regulate NSCLC metastasis in response to ionizing radiation (IR).. The results indicated that MIF and ribosomal protein S3 (rpS3) were shown to be connected to inflammation, proliferation, and metastasis of NSCLC via IR-induced activation of the NF-kB pathway. Article Title: A ligand-induced structural change in fatty acid–binding protein 1 is associated with potentiation of peroxisome proliferator–activated receptor α agonists Article Snippet: The data were processed using the Article Title: Structural and Functional Properties of Tropomyosin Isoforms Tpm4.1 and Tpm2.1 Article Snippet: Tropomyosin (Tpm) is one of the most important partners of actin filament that largely determines its properties.. In animal organisms, there are different isoforms of Tpm, which are believed to be involved in the regulation of various cellular functions.. However, molecular mechanisms by which various Tpm cytoplasmic regulate of the functioning of actin filaments are still poorly understood. Article Title: Capped and uncapped antibody cysteines, and their use in antibody-drug conjugation Article Snippet: The resulting heat capacity was baseline corrected by subtracting against a blank Histidine/sucrose formulation scan and fitted with the non-2 state transitions function using Article Title: The interaction of hyperthermophilic TATA-box binding protein with single-stranded DNA is entropically favorable and exhibits a large negative heat capacity change at high salt concentration. Article Snippet: Molecular BioSystems This article was published as part of the 2009 Emerging Investigators Issue Highlighting the work of outstanding young scientists at the chemical- and systems-biology interfaces Please take a look at the full table of contents to access the other papers in this issue.. Pu bl is he d on 1 3 M ay 2 00 9.. D ow nl oa de d by U ni ve rs ity o f W in ds or o n 28 /1 0/ 20 14 0 3: 43 :1 6. Sampling:Article Title: Impacts of fast production of afucosylated antibodies and Fc mutants in ExpiCHO-S™ for enhancing FcγRIIIa binding and NK cell activation. Article Snippet: The resulting heat capacity was baseline corrected by subtracting against a blank PBS scan and fitted with the non-2 state transitions function using Article Title: A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal. Article Snippet: Accepted Manuscript A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal Wen-Si Yang, Yuan Hong, Ying Zhang, Da-Cheng Wang, De-Feng Li, Yan-Jie Hou PII: S0006-291X(18)30374-7 DOI: 10.1016/j.bbrc.2018.02.143 Reference: YBBRC 39508 To appear in: Biochemical and Biophysical Research Communications Received Date: 14 February 2018 Accepted Date: 16 February 2018 Please cite this article as: W.-S. Yang, Y. Hong, Y. Zhang, D.-C. Wang, D.-F. Li, Y.-J.. Hou, A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal, Biochemical and Biophysical Research Communications (2018), doi: 10.1016/j.bbrc.2018.02.143.. This is a PDF file of an unedited manuscript that has been accepted for publication. Article Title: Effects of dehydroepiandrosterone sulfate on the evoked cortical activity of controls and of brain-injured rats. Article Snippet: Dehydroepiandrosterone (DHEA) and its sulfate (DHEAS) are sex hormone precursors which exert marked neurotrophic and/or neuroprotective activity in the central nervous system (CNS).. In the present electrophysiological experiments, we studied the effects of peripherally administered DHEAS on responses of the primary somatosensory (SSI) and motor cortices (MI) of (i) anesthetized controls and (ii) MI focal cold-lesioned rats. (iii) The effects of DHEAS on the field excitatory postsynaptic potentials (fEPSPs) were also studied in vitro brain slices.. DHEAS (50 mg/kg) was injected subcutaneously 12 h before and immediately after cold lesion induction. Article Title: Dissociation of MIF-rpS3 complex and sequential NF-κB activation is involved in IR-induced metastatic conversion of NSCLC. Article Snippet: Frequent relapse and spreading of tumors during radiotherapy are principal obstacles to treatment of non-small cell lung cancer (NSCLC).. In this study, we aimed to investigate how macrophage migration inhibitory factor (MIF) which is expressed at high levels in metastatic and primary lung cancer cells could regulate NSCLC metastasis in response to ionizing radiation (IR).. The results indicated that MIF and ribosomal protein S3 (rpS3) were shown to be connected to inflammation, proliferation, and metastasis of NSCLC via IR-induced activation of the NF-kB pathway. Article Title: A ligand-induced structural change in fatty acid–binding protein 1 is associated with potentiation of peroxisome proliferator–activated receptor α agonists Article Snippet: The data were processed using the Article Title: Structural and Functional Properties of Tropomyosin Isoforms Tpm4.1 and Tpm2.1 Article Snippet: Tropomyosin (Tpm) is one of the most important partners of actin filament that largely determines its properties.. In animal organisms, there are different isoforms of Tpm, which are believed to be involved in the regulation of various cellular functions.. However, molecular mechanisms by which various Tpm cytoplasmic regulate of the functioning of actin filaments are still poorly understood. Article Title: Capped and uncapped antibody cysteines, and their use in antibody-drug conjugation Article Snippet: The resulting heat capacity was baseline corrected by subtracting against a blank Histidine/sucrose formulation scan and fitted with the non-2 state transitions function using Article Title: The interaction of hyperthermophilic TATA-box binding protein with single-stranded DNA is entropically favorable and exhibits a large negative heat capacity change at high salt concentration. Article Snippet: Molecular BioSystems This article was published as part of the 2009 Emerging Investigators Issue Highlighting the work of outstanding young scientists at the chemical- and systems-biology interfaces Please take a look at the full table of contents to access the other papers in this issue.. Pu bl is he d on 1 3 M ay 2 00 9.. D ow nl oa de d by U ni ve rs ity o f W in ds or o n 28 /1 0/ 20 14 0 3: 43 :1 6. |
![( A ) Affinities of 2F2 Fab for peptides 210-1, 210-2, 210-3, 210-4, and 210-5 as measured by <t>isothermal</t> <t>titration</t> <t>calorimetry</t> (ITC). Open circles represent independent measurements. Mean binding constant (K D ) and binding stoichiometry (N) values are shown above the corresponding bar. Error bars represent SEM. ( B ) Upper panel: sequences of peptides used in ITC experiments with variable residues underlined. Dark gray denotes the core epitope of the peptide resolved in all the X-ray crystal structures, and light gray shading indicates residues resolved in the corresponding X-ray crystal structures. Bottom panel: comparison of the conformations of PvCSP210 peptides in X-ray crystal structures. PvCSPvk210 peptides are colored from navy to light blue, with the residues adopting one turn of a 3 10 -helix depicted in pink. ( C ) Top and side views of the 210-4 peptide (light blue) in the binding groove of the 2F2 Fab shown as surface representation (heavy chain [HC] shown in green and kappa chain [KC] shown in white). ( D ) Comparison of the conformations adopted by the core epitope of peptides 210-1, 210-2, 210-3, 210-4, and 210-5 when bound to 2F2. ( E ) Detailed interactions between Fab 2F2 and peptide 210-4. H-bonds and salt bridges are shown as black dashes, peptide 210-4 is shown in light blue, HC is shown in green, and KC is shown in gray. Fab residues are annotated with H or K letters to indicate heavy and kappa light chain, respectively.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_9896/pmc08809896/pmc08809896__elife-72908-fig3.jpg)